Cell Culture Optimization
BioDOE: Design Smarter Biological Experiments, Accelerate Discovery Faster.
Stop guessing with wasteful One-Factor-at-a-Time (OFAT) trials. BioDOE provides an intuitive 3-Panel Design Studio for assays, bioprocessing, formulations, and multi-response surface optimization without requiring a PhD in statistics.
Built for Biological Complexity
From high-throughput screening and formulation buffers to equipment constraints in bioprocessing suites.
1. Biological Factor Setup
Specify 2-level continuous ranges (pH, Substrate Concentration) and discrete multi-option choices (Media Types, Enzyme Strains, Buffer Components).
Explore Factor Setup2. Split-Plot Constraints
Flag Hard-to-Change equipment settings like *Incubation Temp* or *Bioreactor Vessel* to group runs into physical Whole-Plots without resetting equipment.
Explore Split-Plot Engine3. Multi-Objective Desirability
Derringer-Suich desirability engine optimizing conflicting targets simultaneously (e.g. **Maximize Yield $\uparrow$** while **Minimizing Impurity $\downarrow$**).
Explore 3D Surface MeshAccelerating Discovery Across Every Assay & Bioprocess
Whether optimizing CHO cell culture, Immunoassay Development (ELISA & Potency Assays), or formulation stability, BioDOE reduces experimental runs by up to 75%.
CHO Cell Culture Media Optimization
Simultaneously evaluate Glucose, Amino Acid Feeds, and Temperature Shift timing to boost monoclonal antibody titers while maintaining 95%+ cell viability.
Bioprocess Case StudyImmunoassay & Bioassay Development
Optimize ELISA, HTRF, and Cell-Based Potency Assays. Simultaneously screen antibody coating levels, detection conjugate dilutions, blocking agents, and incubation times to maximize signal-to-noise (S/N) ratio and dynamic range.
Immunoassay OptimizationRecombinant Protein & Enzyme Expression
Screen induction timing, IPTG/Methanol concentration, and Dissolved Oxygen levels. Account for hard-to-change incubator temps via Split-Plot designs.
Expression WorkflowAssay Buffer & Formulation Stability
Formulate high-stability biologics. Optimize pH, ionic strength, surfactants, and cryoprotectants to prevent aggregation through freeze-thaw cycles.
Formulation MatrixGene Therapy & Viral Vector Transfection
Optimize AAV or Lentivirus production by balancing plasmid ratios, transfection reagent concentrations, and harvest timing for maximum viral titer.
Gene Therapy Solutions
"BioDOE transformed our CHO cell culture media optimization. In just 12 runs, we identified critical Glucose and Temperature interactions that we had spent 6 months trying to find with traditional OFAT experiments. A total game-changer for bioprocess development."
Simple Plans Built for Every Stage of Research
Start free, upgrade as your experimental volume grows. No expensive desktop licenses required.
- 1 Free Active DOE Study
- Screening & Characterization Designs
- 1 Free REML ANOVA & Surface Plot
- CSV Data Import & Export
- Supabase Authentication
- Unlimited DOE Design Generation
- Unlimited REML MixedLM & ANOVA
- Split-Plot Bioreactor Constraints
- Derringer-Suich Multi-Target Desirability
- Publication High-Res PNG & Vector SVG
- Supabase Cloud Storage Sync
Ready to Transform Your Biological Optimization?
Join leading life scientists, bioprocess engineers, and biotech researchers using BioDOE to design, execute, and optimize multi-factor experiments in minutes.
1. Define Factors & Responses
| Factor Name | Values / Range |
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| Response Name | Optimization Goal |
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2. Design of Experiment
| # |
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3. Data Analysis
| Factor | p-value | Sig |
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